![]() ![]() ![]() By simulating the process of sampling barcodes from the viral library, ratio of re-used barcodes can be estimated. Here the plot shows the effect of viral barcode threshold on the percentage of removed barcodes from BRICseq data (BL6-1), and the re-used ratio in the residual barcodes. To reduce re-used barcode errors, barcodes whose counts were greater than a threshold (viral abundance threshold) in batch 1 of viral library 2 were excluded for analysis in BRICseq data. Distribution of barcode counts in batch 2 of viral library 2. Distribution of barcode counts in batch 1 of viral library 2. Typical experiments performed for this study will have ~600 projection cells per cubelet (shaded region), resulting in a probability of unique barcodes equal to 92.6 and 99.8%, depending on whether the intermediate or high diversity viral library is used. ![]() The ratio of projection barcodes to ‘non-projection’ barcodes (see STAR Methods section ‘correction of re-used barcodes’) in BL6-1 was used for calculation. ![]() We assume 120 source cubelets are infected for this analysis, and the barcodes in the viral library are uniformly distributed. The diversity of viral barcodes needed for unique labeling. Details are discussed in STAR Methodssection ‘correction of re-used barcodes’. If the same barcode is used more than once to infect multiple neurons, somata of some of the infected neurons are mis-interpreted as axons. Re-used barcode is another error source in BRICseq. Increasing UMI threshold helped further reduce errors caused by template switching. Cubelets from a different BRICseq brain were added during PCR to estimate template switching rates. In BL6-1, PCR was performed in a 12 mL system. BRICseq was performed with samples from two brains, and 96 cubelets (48 from each) were pooled together for PCR and sequencing. Increasing the PCR volume from 25μL to 2mL dramatically decreased template switching rates. Details are discussed in STAR Methods section ‘correction of template switching’. During PCR, a DNA strand being synthesized may use a new template during elongation, due to a shared sequence among all the templates. Template switching is one error source in BRICseq. Distribution of the number of single cell projection targets in BL6-1. The circles indicate the location of the source cubelet. Dorsal view of projections of 3 example cells in cubelet 53 in BL6-1. Cells are clustered by non-negative matrix factorization (10 clusters). Each row is one cell, and each column is a target cubelet. Single cell projection patterns from 712 cells residing in an example cubelet (cubelet 53, in PTLp) in BL6-1. ![]()
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![]() ![]() DeMille erected 21 giant sphinxes and an 800-foot-wide temple as a set for the silent, black-and-white classic movie "The Ten Commandments."īut in 1923, when filming was over, DeMille abandoned them there among the sands of the Guadalupe-Nipomo Dunes in Santa Barbara County. More than 90 years ago, legendary filmmaker Cecile B. Archaeologists working in the sand dunes along the Central California coast are digging up ancient sphinxes - but these are made of plaster. "The Ten Commandments (2 stars out of 5)". " '30 Days of Night' Leads Lifeless Crowd".
![]() ![]() Â Implementing Minimum Viable Experience (MVE) ![]() To underline the target market the app was first launched in select countries in Asia and Africa (Bangladesh, Nepal, Nigeria, South Africa, Sudan, Sri Lanka, Vietnam and Zimbabwe) It is a streamlined version of the social network optimized for low-end Android devices and slow and intermittent internet connections, both common in the developing world. In January 2015 Facebook launched a new Android app called Facebook Lite. Januby tobystereo on UX Facebook Lite Exemplifies Minimum Viable Experience Facebook Lite VDMX and Touchdesigner are always the most popular tools.TOBYSTEREO I'm a digital artist working at the intersection of art and technology, currently Senior Senior UX Designer at Spreadshirt. Also, it is great to build up custom control surfaces for custom needs. According to those needs we are using VDMX or Touchdesigner as a hub to send and receive all the external data to process our visual content for live performances. ) takes a big part in our artistic workflow. Using musical data and external tools ( music instruments, HID devices, sensors etc. Our visuals are mostly geometric and abstract compositions inspired by nature and cosmic pattern. VDMX has a great integration with Quartz Composer and lets us program custom plugins, visuals, FX and templates for our installations and live performances. ![]() Our sound is allways composed and created by our self.Īdditional we can add light with DMX or artnet into our immersive evironments. We use video footage only for very specific reasons. Generally, our live setup contains generative visual patches programmed with Quartz Composer or Interactive Shader Format. ![]() Then we do all the live sequencing, adding video filters and connecting audio, OSC and MIDI signals via VDMX. For most of our audio-visual performances and installations, we are programming our visual content with ISF. We use Mac Book Retina laptop, our own build Hackintosh or Mac pro for programming and live performances, Black Magic Intensity Shuttle for capturing video and live mixing and finally for audio-visual works we use several capture sound card for audio and MIDI input. We are using Quartz Composer, ISF, Touch Designer, Cinema 4D, After FX and VDMX for creating our visual content. Signature Brand Visuals will translate your corporate branding in a creative motion graphic that suits your event & delivers a new level of brand experience. A stage, car, a building or even an island. With Projection Mapping we cover a real life object with projected motion graphics. The composed sounds direct the visual landscape. LiCHTPiRATEN creates immersive multimedia environments and visual systems generated in real time. In the end, the visual and auditory space will be different each time – we start with a basic concept and form, but the compositional live aspect is a factor that depends on the atmosphere and emotions. In other words, our performances are strongly based on a constant bi-directional feedback process between sound- and lightwaves. On the other hand, music and sounds are informed/affected by the way things look, also in real time. If, for example, dynamics, pitch or articulation in the sound change, there will be a real-time translation into visible forms, shapes or colors. Very important to us is the process of finding visual analogies of musical and sound parameters. Sometimes the scenes tell a story, sometimes it is rather abstract, really depending on the purpose of our presence and the atmosphere that we want to create. This concept is more or less based on different scenes. LiCHTPiRATEN is a group of visual artists, composers and programmers,Ĭhallenging the barriers between reality and perception.Īll our work is intended to be live-performance.īefore each show/event, we create a concept for the visual environment as well as a composition for the music and sounds that we want to use. ![]() ![]() ![]() After unlocking Ninjoy, the game announces, "Get a gold award on each customer to unlock a special customer!", that 'special customer' being Papa Louie. (Though Papa Louie is not a Closer, he also is not unlocked like a normal character. Papa Louie (Unlocked when all customers, closers, and the Food Critic have gold Customer Awards).While Cactus McCoy took first place in the Action-Platform category, Freezeria finished second in the Simulation or Strategy category. On January 13, 2012, Tony Solary revealed that Papa's Freezeria and Cactus McCoy and the Curse of Thorns, were both nominated for "Best of 2011" awards by Jay-is-games. Wallpapers and flooring are introduced as well, allowing the player to change the design of the store itself.The lobby is now customizable, allowing a player to interchange the furniture or posters purchased at the store.Upon arrival, all of Papa Louie's customers exit the ship, and head over to the Freezeria, much to his/her dismay. Upon taking a closer look, he/she sees that the cruise ship S.S. Later, Alberto/Penny sees what looks to be a ship in the distance. Papa then leaves him/her for his next adventure. Now wearing the official uniform, Papa Louie gives him/her a tour of his new franchise. The manual indicates "Stress-Free Work", and workers can decorate the store as he/she wishes (provided that they pay for it themselves in fine print). Go for a Tiki Island theme, add bookshelves and coffee stands, fill the room with jukeboxes - the choice is yours!Īlberto/ Penny is reading the training manual for his/her new job at Papa's Freezeria, Papa Louie's new restaurant on Calypso Island. Papas Pancakeria at Cool Math Games: Use counting and time management to run a pancakeria. You can place decorations any way you like in your lobby, letting you fully customize your shop with wallpaper, posters, furniture and more. New to the series, the sundae shop features over 100 lobby decorations that you can purchase in the Furniture Shop, with each item affecting your scores while playing. Challenging "Closer" customers and the Food Critic return from "Papa's Taco Mia!", as well as weekly paychecks, upgrades, and 80 in-game Badges to earn while serving ice cream. The time-management and hands-on gameplay of the series is back, with new challenges related to blending ingredients and serving the right amount of ice cream in sundae cups. This latest game features dozens of syrups, candies, and toppings that unlock as you play, and a variety of new and returning customers looking to beat the heat with your ice cream sundaes. Boat loads of customers are coming to Calypso Island in search of the best Freezer treats around. That is until Papa Louie takes off, leaving you in charge during the peak of tourist season. Papa's Freezeria is an ocean front ice cream shop that will surely be a relaxing way to spend the summer. You've just landed an amazing summer job on the tropical island of Calypso. Beat the Summer Heat with Papa's Freezeria! ![]() |
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